Gene

FLT3

fms related receptor tyrosine kinase 3

Explore in graph →CI-GENE-00002044HGNC:3765 ENSG00000122025 NCBI 2322 CIViC Cancer geneApproved

Curated evidence

Clinical evidence (266)

CIViC items involving this gene, grouped by molecular profile and therapy, with native levels and directions. 50 items per page.

civicCuratedShowing 51–100 of 266 evidence items · levels, directions and significance as curated at the source; each row links to its CIViC record.

Therapy	Cancer	Type	Level	Direction · significance	Rating (1–5)	Status	Evidence	Source
FLT3 D835Y6
Lestaurtinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Sensitivity Response	4	accepted

Cohorts

Alteration frequency by cohort (125)

Frequency = cases affected / cases profiled within one cohort. Cohorts are never pooled.

cbioportalObserved125 cohort rows (showing 50, sorted by frequency) · frequency = affected / profiled, as published by each cohort; cohorts are not pooled. Hover a row badge for its release.

Cohort	Mapped cancer	Alteration	Affected (n)	Profiled (n)	Frequency (%)	Rank in cohort	Source
Acute Myeloid Leukemia (OHSU, Cancer Cell 2022) aml_ohsu_2022	Acute Myeloid Leukemia	Ssm	278

Literature

Linked publications (46)

25 per page, newest first.

pubmedPublishedShowing 1–25 of 46 publications · bibliographic data as recorded by PubMed; abstracts are not reproduced.

Gilteritinib or Chemotherapy for Relapsed or Refractory FLT3-Mutated AML.
· N Engl J Med · 2019 · PMID 31665578 · validated civic_curation pubmed
Prognostic significance of FLT3 internal tandem duplication, nucleophosmin 1, and CEBPA gene mutations for acute myeloid leukemia patients with normal karyotype and younger than 60 years: a systematic review and meta-analysis.
· Ann Hematol · 2014 · PMID 24801015 · validated civic_curation pubmed
Midostaurin approved for FLT3-mutated AML.
· Blood · 2017 · PMID 28546144 · validated civic_curation pubmed
DNMT3A mutations in acute myeloid leukemia.
· N Engl J Med · 2010 · PMID 21067377 · validated civic_curation pubmed

FLT3 D839A1
Pexidartinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8671 Mutations were identified by in vitro saturation mutagenesis on the background of FLT3-ITD or FLT3-ITD/F691L. Proliferation and biochemical studies showed that the FLT3-ITD D839A (IC50 = 4.0μM) and FL… (full text at CIViC) PMID 25847190 · Smith et al., 2015 · Open in CIViC	civic

FLT3 D839A1

Pexidartinib

Acute Myeloid LeukemiaCURATED_BROADER

Predictive

Supports Resistance

submitted

EID8671

Mutations were identified by in vitro saturation mutagenesis on the background of FLT3-ITD or FLT3-ITD/F691L. Proliferation and biochemical studies showed that the FLT3-ITD D839A (IC50 = 4.0μM) and FL… (full text at CIViC)

PMID 25847190 · Smith et al., 2015 · Open in CIViC

FLT3 D839G3
(prognostic)	Acute Promyelocytic Leukemia	Prognostic	C	Supports Poor Outcome	1	submitted	EID8908 This is a case report about an adult patient with acute promyelocytic leukemia (APL) who had relapse in a testicle prior to systemic relapse of APL. Retrospective analysis of the preserved samples fro… (full text at CIViC) PMID 23816818 · Zou et al., 2013 · Open in CIViC	civic
(oncogenic)	Acute Myeloid LeukemiaCURATED_BROADER	Oncogenic	C	Supports Oncogenicity	1	submitted	EID8927 Single strand polymorphism targeting FLT3 in addition to RUNX1, KIT, CEBPA, PTPN11, and NRAS was carried out to identify novel recurring gene mutations in an acute myeloid leukemia cohort comprised of… (full text at CIViC) PMID 15667533 · Smith et al., 2005 · Open in CIViC	civic
(functional)	—UNRESOLVED	Functional	D	Supports Gain Of Function	3	submitted	EID8656 Functional evaluation of FLT3-D839G was performed by analyzing proliferation, apoptosis, receptor alteration and signaling in vitro. When compared to FLT3-WT expressing cells, FLT3-D839G showed signif… (full text at CIViC) PMID 24608088 · Janke et al., 2014 · Open in CIViC	civic

FLT3 E588_Y589INSKYFYVDFRE4
Palbociclib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Sensitivity Response	—	submitted	EID4738 In an in vitro study, MOLM-14, PL21 and MV4-11 cell lines expressing FLT3 internal tandem duplication mutation (FLT3-ITD) demonstrated increased sensitivity to palbociclib treatment, compared to THP-1… (full text at CIViC) PMID 27099147 · Uras et al., 2016 · Open in CIViC	civic
Sunitinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	C	Supports Sensitivity Response	—	submitted	EID4735 In a retrospective study of 15 acute myeloid leukemia patients, the patients with FLT3 internal tandem duplication mutation (n=2) were associated with improved response to sunitinib treatment. The pat… (full text at CIViC) PMID 15459012 · Fiedler et al., 2005 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	C	Supports Sensitivity Response	—	submitted	EID4736 In a phase I study of 29 acute myeloid leukemia patients, patients with FLT3 internal tandem duplication (ITD) mutation (n=3) were associated with improved response to sunitinib monotherapy compared … (full text at CIViC) PMID 14654525 · O'Farrell et al., 2003 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Sensitivity Response	—	submitted	EID4737 In an in vitro study, leukemia M4;11 (endogenous), myeloid 32D (overexpression) and Chinese hamster ovary (transient expression) cell lines expressing FLT3 internal tandem duplication mutation demonst… (full text at CIViC) PMID 12531805 · O'Farrell et al., 2003 · Open in CIViC	civic

FLT3 F691L13
Crenolanib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	5	submitted	EID12387 FLT3 F691L was observed in 2 patient samples collected after at least 28 days of treatment with crenolanib. Prior to treatment with crenolanib, both patients were treated with quizartinib. Ba/F3 cells… (full text at CIViC) PMID 30651561 · Zhang et al., 2019 · Open in CIViC	civic
FLT3/ABL/Aurora Kinase Inhibitor KW-2449	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8846 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in FLT3 TKIs identified F691L. Cytotoxicity assessed by MTT demonstrated Ba/F3 cells expressing FLT3/IT… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic
Gilteritinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8562 With Ba/F3 cells bearing FLT3-ITD F691L mutations and by in vitro proliferation assay, it was found that the cells exhibited >10-fold resistance to Gilteritinib. Similar resistance was found in cells … (full text at CIViC) PMID 32040554 · Tarver et al., 2020 · Open in CIViC	civic
Lestaurtinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	accepted	EID8341 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in FLT3 TKIs identified F691L. Cytotoxicity assessed by MTT demonstrated Ba/F3 cells expressing FLT3/IT… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic
Midostaurin	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	accepted	EID8342 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in the FLT3 TKI midostaurin identified F691L in 1 of 400 clones. Cytotoxicity assessed by MTT demonstra… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic
Pexidartinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	B	Supports Sensitivity Response	3	submitted	EID8674 Deep sequencing revealed that no secondary F691L mutations were found in all 9 patients who achieved a partial or complete response on PLX3397 and who subsequently relapsed on the drug, suggesting thi… (full text at CIViC) PMID 25847190 · Smith et al., 2015 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	B	Does Not Support Resistance	1	rejected	EID9716 Overall response rate was 21% in this clinical trial with Pexidartinib on relapsed or refractory AML patients with FLT3-ITD. Forty two out of the total 80 patients were tested for FLT3 KD mutations be… (full text at CIViC) PMID 32330242 · Smith et al., 2020 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	B	Does Not Support Resistance	1	submitted	EID9717 Overall response rate was 21% in this clinical trial with Pexidartinib on relapsed or refractory AML patients with FLT3-ITD. Forty two out of the total 90 patients were tested for FLT3 KD mutations be… (full text at CIViC) PMID 32330242 · Smith et al., 2020 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Sensitivity Response	3	submitted	EID8664 Proliferation studies revealed that PLX3397 has the ability to inhibit the proliferation of Ba/F3 cells expressing FLT3-ITD/F691L (IC50 = 0.097μM). PLX3397 equally inhibited the proliferation of MOLM1… (full text at CIViC) PMID 25847190 · Smith et al., 2015 · Open in CIViC	civic
Quizartinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	B	Supports Resistance	3	submitted	EID9718 Using AC220 (Quizartinib) treatment in Ba/F3 cell lines in a in vitro saturation mutagenesis assay, variants in four residues in FLT3 kinase domain were found, including E608, F691, D835 and Y842. Exc… (full text at CIViC) PMID 22504184 · Smith et al., 2012 · Open in CIViC	civic
〃	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8843 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in FLT3 TKIs identified F691L. Cytotoxicity assessed by MTT demonstrated Ba/F3 cells expressing FLT3/IT… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic
Sorafenib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8845 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in FLT3 TKIs identified F691L. Cytotoxicity assessed by MTT demonstrated Ba/F3 cells expressing FLT3/IT… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic
Sunitinib	Acute Myeloid LeukemiaCURATED_BROADER	Predictive	D	Supports Resistance	3	submitted	EID8844 A screen using saturation mutagenesis of FLT3/ITD followed by selection of transfected TF1 cells in FLT3 TKIs identified F691L. Cytotoxicity assessed by MTT demonstrated Ba/F3 cells expressing FLT3/IT… (full text at CIViC) PMID 22858906 · Williams et al., 2013 · Open in CIViC	civic

FLT3 G73E3
(oncogenic)	Malignant NeoplasmALIAS	Oncogenic	C	Does Not Support Oncogenicity	2	submitted	EID12425 The variant has been identified in the following tumors. Tumors from the AACR GENIE Project are likely somatic, but most testing was unmatched with a germline sample (https://www.aacr.org/wp-content/… (full text at CIViC) PMID 37668528 · de Bruijn et al., 2023 · Open in CIViC	civic
〃	Chronic Myeloid Leukemia, BCR-ABL1 PositiveALIAS	Oncogenic	C	Supports Oncogenicity	2	rejected	EID12426 The variant was identified by NGS in a CML (unmatched analysis) (Supplementary File 2). PMID 32943879 · Wu et al., 2020 · Open in CIViC	civic
〃	Basal Cell Carcinoma	Oncogenic	C	Supports Oncogenicity	1	submitted	EID12476 A set of samples from 293 BCCs were sequenced. 263 were sporadic cases and 30 were Gorlin syndrome. 1 sample from patient ID VS069 had FLT3 c.218G>A resulting in G73E. Thie patient sporadic BCC, and w… (full text at CIViC) PMID 26950094 · Bonilla et al., 2016 · Open in CIViC	civic