Publication
Saturation mutagenesis identifies activating and resistance-inducing FGFR kinase domain mutations.
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- PubMed
- Retrieved
- Sep 8, 2026
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- normalized (units and labels harmonized; values unchanged)
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- ING-CIVIC-20260908-000001
Abstract
Abstract (excerpt)
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Linked entities
Linked entities (4)
How each link was made (MeSH, dictionary, registry reference, curation…) and whether it has been validated. Candidate links are not counted in entity statistics.
Validated 4
- cancerMalignant Neoplasmcivic_curation1.00
- geneFGFR3civic_curation1.00
- variantFGFR3 A500Tcivic_curation1.00
- variantFGFR3 N540Kcivic_curation1.00
Curated evidence
Evidence citing this paper (26)
- Source
- CIViC — Clinical Interpretation of Variants in Cancer
- Dataset
- CIViC evidence items
- Version
- civic-2026-09-08
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Evidence
- expert curation
- License
- CC0 1.0
- PMID
- 41361008
- Run
- ING-CIVIC-20260908-000001
| Therapy | Cancer | Type | Level | Direction · significance | Rating (1–5) | Status | Evidence | Source |
|---|---|---|---|---|---|---|---|---|
| FGFR2 C491S1 | ||||||||
| Futibatinib | Lung Non-Small Cell CarcinomaCURATED_BROADER | Predictive | D | Supports Resistance | 4 | submitted | EID13173FGFR2 C491S was identified as selectively conferring futibatinib resistance in FGFR-dependent NCI-H1581 cells and was independently validated by drug-treated cell-viability and FGFR phosphorylation ex… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 V564G1 | ||||||||
| Pemigatinib | Lung Non-Small Cell CarcinomaCURATED_BROADER | Predictive | D | Supports Resistance | 4 | submitted | EID13172In FGFR-dependent NCI-H1581 lung cancer cells, FGFR2 V564G was identified by saturation mutational scanning as a pemigatinib-resistance mutation and was independently validated by cell-viability and p… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 A500T1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Does Not Support Oncogenicity | 2 | accepted | EID13092In a saturation mutagenesis study, all possible single nucleotide substitutions in the FGFR1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 D786N1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12894In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 D786Y1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12895In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 I538V1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12879In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 K649R1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12887In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 K650E1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 2 | submitted | EID12889In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 K650M1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12890In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 K650Q1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12888In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 K650T1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12891In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 L645H1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12884In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 L645R1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12886In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 M528I1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 2 | submitted | EID12878In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 N540K1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 2 | accepted | EID12881In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 N540S1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12880In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 R669G1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 2 | submitted | EID12892In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 S782P1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12893In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 V555L1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 2 | submitted | EID12883In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 V555M1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Supports Oncogenicity | 1 | submitted | EID12882In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR3 Y647C1 | ||||||||
| (oncogenic) | Malignant NeoplasmALIAS | Oncogenic | D | Does Not Support Oncogenicity | 2 | submitted | EID13202In a saturation mutagenesis study, all possible point mutations in the FGFR 1-4 kinase domain (amino acids 472-807 for FGFR3) were tested. Lentiviral plasmids were infected at MOI < 0.3 into the growt… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 A726D1 | ||||||||
| (functional) | —UNRESOLVED | Functional | D | Supports Gain Of Function | 4 | submitted | EID13169FGFR2 A726D was identified as an activating mutation by saturation mutational scanning and independently demonstrated increased FGFR/FRS2 phosphorylation, growth under low-serum conditions, and anchor… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 E711K1 | ||||||||
| (functional) | —UNRESOLVED | Functional | D | Supports Gain Of Function | 3 | submitted | EID13171FGFR2 E711K was identified as activating in the saturation mutational screen and independently demonstrated increased FGFR and FRS2 phosphorylation compared with wild-type FGFR2 in MCF10A cells, suppo… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 I654K1 | ||||||||
| (functional) | —UNRESOLVED | Functional | D | Supports Gain Of Function | 4 | submitted | EID13167FGFR2 I654K was identified as an activating mutation by saturation mutational scanning and was independently validated in MCF10A and NIH-3T3 models. FGFR2 I654K increased FGFR/FRS2 phosphorylation rel… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 I654R1 | ||||||||
| (functional) | —UNRESOLVED | Functional | D | Supports Gain Of Function | 4 | submitted | EID13168FGFR2 I654R was identified as an activating kinase-domain mutation in the functional screen and independently evaluated in NIH-3T3 cells, where it supported proliferation under low-serum conditions re… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |
| FGFR2 N727D1 | ||||||||
| (functional) | —UNRESOLVED | Functional | D | Supports Gain Of Function | 4 | submitted | EID13170FGFR2 N727D was classified as activating by saturation mutational scanning and independently validated by increased FGFR/FRS2 phosphorylation, proliferation under low-serum conditions, and anchorage-i… (full text at CIViC) PMID 41361008 · Tangermann et al., 2025 · Open in CIViC | civic |