Mechanism of activation of the RAF-ERK signaling pathway by oncogenic mutations of B-RAF.

The BRAF mutation G463E resulted in elevated kinase activity relative to wild-type BRAF in vitro. B-RAF activity was determined using kinase dead MEK as a substrate. G463E activity was 28 times higher… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF G463V protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF G463V had a basal kinase activity 46 times higher than that of… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF G465A protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF G465A had a basal kinase activity 5 times higher than that of … (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF G468A protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF G468A had a basal kinase activity 266 times higher than that o… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF N580S protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF N580S had a basal kinase activity 7 times higher than that of … (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF T598I protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF T598I had a basal kinase activity 30 times higher than that of… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF V600E (also known as V599E in the paper) protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF V600E had a basal kinase ac… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

In this study, myc-tagged BRAF V600E (reported as V599E) transiently expressed in COS cells exhibited approximately 478-fold greater basal kinase activity than wild-type BRAF, constitutively activated… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

The BRAF mutation A728V (also known as A727V) resulted in elevated kinase activity relative to wild-type BRAF in vitro, as well as increased phosphorylation of ERK1/2 in COS cells. B-RAF activity was … (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF E585K protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF E585K had a basal kinase activity 129 times higher than that o… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF F594L protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF F594L had a basal kinase activity 60 times higher than that of… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

BRAF p.Gly469Ala (NM_004333.6:c.1406G>C) causes increased MEK phosphorylation, increased ratio of pERK/ERK. Class 2 BRAF variant. (In PMID: 15035987 AA numbers shifted by one, AA 468 corresponds to AA… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

BRAF p.Gly469Glu (NM_004333.6:c.1406G>A) causes increased MEK phosphorylation, increased signaling through RAF1 (CRAF), it has low kinase activity. Class 3 BRAF variant. (In PMID: 15035987 AA numbers … (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF I462S protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF I462S had a basal kinase activity 11 times higher than that of… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF K600E protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF K600E had a basal kinase activity 138 times higher than that o… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

BRAF p.Lys601Glu (NM_004333.6:c.1801A>G) causes increased pERK/ERK ratio.

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF L596V protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF L596V had a basal kinase activity 64 times higher than that of… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF R461I protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF R461I had a basal kinase activity 6 times higher than that of … (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF V599R protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF V599R had a basal kinase activity 244 times higher than that o… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF V600D (also known as V599D in the paper) protein in COS cells and measuring its direct MEK phosphorylation activity, the experiment showed that B-RAF V600D had a basal kinase activ… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

By expressing B-RAF V600K (also known as V599K in the paper) protein in COS cells and measuring its direct MEK phosphorylation activity, these experiments showed that B-RAF V600K had a basal kinase ac… (full text at CIViC)

PMID 15035987 · Wan et al., 2004 · Open in CIViC

BRAF p.Val600Lys (NM_004333.6:c.1798_1799delinsAA) causes increased pERK/ERK ratio, and increased MEK phosphorylation. Class 1 BRAF variant.

PMID 15035987 · Wan et al., 2004 · Open in CIViC