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Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethylation.

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Blood2011PMID 21190999PMC3062411stubpubmedProvenance
Source
PubMed
Retrieved
Sep 8, 2026
Layer
normalized (units and labels harmonized; values unchanged)
Run
ING-CIVIC-20260908-000001
Published

Abstract

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Linked entities

Linked entities (5)

How each link was made (MeSH, dictionary, registry reference, curation…) and whether it has been validated. Candidate links are not counted in entity statistics.

Validated 5

Curated evidence

Evidence citing this paper (7)

civicProvenance
Source
CIViC — Clinical Interpretation of Variants in Cancer
Dataset
CIViC evidence items
Version
civic-2026-09-08
Retrieved
Sep 8, 2026
Layer
normalized (units and labels harmonized; values unchanged)
Evidence
expert curation
License
CC0 1.0
PMID
21190999
Run
ING-CIVIC-20260908-000001
Open at source
CuratedShowing 1–7 of 7 evidence items · levels, directions and significance as curated at the source; each row links to its CIViC record.
TherapyCancerTypeLevelDirection · significanceRating (1–5)StatusEvidenceSource
EZH2 Y646F3
(functional)—UNRESOLVEDFunctionalDSupports Gain Of Function3rejected
EID9516

H3K27me3 level was higher in EZH2 (Y641F) bearing GCB-derived lymphoma cell lines and tumors than in wild-type cells. Expression of Y641F mutation in HEK293T cells with wild-type EZH2 led to an increa… (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
〃—UNRESOLVEDFunctionalDSupports Gain Of Function3accepted
EID9517

H3K27me3 (histone H3 Lys-27–specific trimethylation) level was higher in EZH2 (Y641F) bearing germinal center B-cell type DLBCL derived lymphoma cell lines and tumors than in wild-type cells. There wa… (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
〃—UNRESOLVEDFunctionalDSupports Loss Of Function3accepted
EID9519

A 3H methyl incorporation assay tested the function of EZH2 Y641F containing PRC2 complexes in vitro. It showed that Y641F/N PRC2 complexes had significantly decreased enzyme activity on unmethylated … (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
EZH2 Y646H1
(functional)—UNRESOLVEDFunctionalDSupports Gain Of Function3accepted
EID9521

Whole-cell lysates from frozen GCB-derived lymphoma sections with EZH2 Y641H showed a higher level of H3K27me3 (histone H3 Lys-27–specific trimethylation) compared with those from tumor with wild-type… (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
EZH2 Y646N2
(functional)—UNRESOLVEDFunctionalDSupports Gain Of Function3accepted
EID9518

H3K27me3 (histone H3 Lys-27–specific trimethylation) level was higher in EZH2 (Y641N) bearing germinal center B-cell type DLBCL derived lymphoma cell lines and tumors than in wild-type cells. There wa… (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
〃—UNRESOLVEDFunctionalDSupports Loss Of Function3accepted
EID9520

A 3H methyl incorporation assay tested the function of EZH2 Y641N containing PRC2 complexes in vitro. It showed that Y641F/N PRC2 complexes had significantly decreased enzyme activity on unmethylated … (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic
EZH2 Y646S1
(functional)—UNRESOLVEDFunctionalDSupports Gain Of Function3accepted
EID9522

Whole-cell lysates from frozen GCB-derived lymphoma sections with EZH2 Y641H showed a higher level of H3K27me3 (histone H3 Lys-27–specific trimethylation) compared with those from tumor with wild-type… (full text at CIViC)

PMID 21190999 · Yap et al., 2011 · Open in CIViC

civic