Identification of driver and passenger mutations of FLT3 by high-throughput DNA sequence analysis and functional assessment of candidate alleles.

FLT3 M737I variant was found in patient at diagnosis but absent in the remission sample. BaF3 cells was transformed to express M737I and these cells were unable to grow in the absence of IL-3 similar … (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC

The FLT3 V592G variant was found in a patient with AML. BaF3 cells transformed to express V592G demonstrated proliferation in the absence of IL-3 similar to D835Y positive control. Cell proliferation … (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC

The paper sequenced FLT3 in 222 adult patients with AML and no known FLT3 ITD, KIT, and NRAS mutations. Nine nonsynonmyous changes were identified including one patient with the Y572C amino acid subst… (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC

Ba/F3 cell lines engineered to express Y572C showed sustained growth in the absence of IL-3 similar to the D835Y expressing cell line (positive control) (Figure 1A). The Ba/F3 WT expressing cell line… (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC

Ba/F3 cell lines engineered to express Y572C showed constitutive phosphorylation of FLT3 (Figure 1B) and phosphorylation of other downstream signaling molecules such as ERK1/2, AKT (Figure 2). Based o… (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC

Ba/F3 cell lines engineered to express Y572C showed reduced cell growth when exposed to different concentrations of PKC412 (also known as midostaurin )[IC50 (nm) 2.5] (Figure 4A) when compared to a Y5… (full text at CIViC)

PMID 18068628 · Fröhling et al., 2007 · Open in CIViC