Clinical trial · Observational
Effect of Long-time Human Sperm Storage in Liquid Nitrogen on Semen Parameters
Impact of Storage Time in Liquid Nitrogen on Cryopreserved Human Spermatozoa
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
Sperm cryopreservation is an essential tool for men fertility preservation in the context of gonadotoxic treatments or/and pathologies such as cancers, gamete donation and ART. Nevertheless, it is validated that the freezing and thawing procedures affect sperm parameters and in particular motility. It is therefore essential to determine the impact of storage time on motility and particularly the number of progressive motile spermatozoa which will determine the choice of ART technique. However, few studies have analyzed the impact of storage time in liquid nitrogen and no study over a long period on human spermatozoa and their use in ART. The aim of this study is to assess the impact of long-time storage, from 2 to 12 years, in liquid nitrogen on standard semen parameters, notably motility.
Conditions
Conditions (4)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Cancer | Malignant Neoplasm | ALIAS | 0.90 |
| Cryopreservation | — | UNRESOLVED | — |
| Healthy | — | UNRESOLVED | — |
| Infertility, Male | — | UNRESOLVED | — |
Interventions
Interventions (0)
Data not yet available
Design
Arms and outcomes
Arms (2)
- label
- Group 1
- description
- Same semen samples are analyzed in group 1 and 2. Group 1 correspond to ejaculate semen samples analyzed the day of slow-freezing
- label
- Group 2
- description
- Same semen samples are analyzed in group 1 and 2. Group 2 correspond to the same ejaculate semen samples as group 1 but analyzed after a storage time from 2 to 12 years in liquid nitrogen.
Primary outcomes (1)
- measure
- To evaluate and compare the total and progressive mobility of human spermatozoa post-freezing and post-storage in High Security straws.
- timeFrame
- 07/2022 to 03/2023
- description
- The percentage of total and progressive motile human spermatozoa were assessed according to WHO, 2010 guideline.
Secondary outcomes (2)
- measure
Eligibility
Eligibility (as posted)
- Sex
- Male
- Minimum age
- 19 Years
- Maximum age
- 63 Years
Show eligibility criteria text
Inclusion Criteria: * The ejaculates were frozen in high security straws by slow freezing, * At least two straws per sample were available * The "post-freezing" thawing test was carried out on the day of freezing * The "post-freezing" thawing test carried out at T0 includes cell numbering, vitality and mobility * There is the informed signature of the consent to donate the straws to Research by the patient Exclusion Criteria: * Ejaculate not donated to research, lack of consent to donation to research * Frozen ejaculate in microdrop * Thawing test not carried out or incomplete
References
Publications (0)
Data not yet available