Neoantigen Derived DCs as Cancer Treatment

Inclusion Criteria:

* ≥20 years of age
* Provide written informed consent
* Histologically confirmed stage IV pancreatic cancer, liver cancer, biliary tract cancer and colorectal cancer (excluding sarcoma and neuroendocrine tumor) that refractory or intolerance to standard therapies for their condition (there is no effective treatment by investigator judgement)
* Completed tumor and germline DNA and RNA sequencing and the neoantigen prediction
* Patients with chronic hepatitis B is eligible if receiving anti-hepatitis B agents and the HBV DNA level \< 2000 IU/ml prior to the preparation phase. Patients with chronic hepatitis C are eligible if HCV RNA is undetectable (\<15 IU/ml) prior to the preparation phase
* Adequate organ function

  * Absolute neutrophil count \>1000/mcL
  * Hemoglobin \> 8.0 g/dl
  * Platelet \> 50000/mcL
  * PT/aPTT \< 1.5 x upper limit of normal (ULN)
  * AST/ALT \< 3 x ULN
  * Bil(T) \< 1.5 x ULN
  * BUN/Cr \< 1.5 x ULN
* Adequate immune system as defined by

  * IgG \> 614 mg/dl
  * IgM \> 53mg/dl
* Eastern Cooperative Oncology Group (ECOG) performance status 0, 1, or 2
* Life expectancy at least\>12weeks
* At least one measurable target lesion as defined by RECIST 1.1

Exclusion Criteria:

* Sarcoma、neuroendocrine tumor
* Last anticancer therapy administered within 2 weeks and any AEs should be ≤ grade 2 prior to leukapheresis
* Patients who cannot tolerate leukapheresis and follow-up blood sampling of 50ml at day 43, day 85 and end-of- treatment.
* Any known active infection as judged by the investigator
* Any known chronic active infection of HIV, HTLV-1 or HTLV-2
* Requirement of systemic treatment with either corticosteroids (\> 10 mg daily prednisone equivalents) or other immunosuppressive medications within 14 days prior to the screen phase. Inhaled or topical steroids and adrenal replacement doses \> 10 mg daily prednisone equivalents are permitted in the absence of active autoimmune disease.
* Other immunocompromising condition that in the opinion of the treating physician renders the patient a poor candidate for this trial
* Pregnant women, nursing women, men or women of childbearing potential who are unwilling to employ adequate contraception (condoms, diaphragm, birth control pills, injections, intrauterine device (IUD), abstinence, etc.)
* Patients with history of penicillin allergy
* Other medical problems or conditions that, in the opinion of the investigator, would make participation in the study hazardous for the patient