Clinical trial · Observational
Establishment and Application of 3-Dimensional (3-D) Cell Culture Model of Clinical Circulating Tumor Cells (CTCs)
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
The goal of this observational study is to validate and evaluate the clinical feasibility of using a 3-D cell culture model for CTCs isolation/purification and their cell number expansion in cancer patient with transitional cell carcinoma and patient without cancer. This project first aims to study the clinical feasibility of utilizing a 3-D cell culture model for the isolation/purification of all possible CTCs in a blood sample in a label-free, viable, and high-purity manner. Through 3-D CTC culture, moreover, the cell number of CTCs can be adequately expanded. All these advantageous features are beyond what is currently possible by using the existing methods. In addition, the harvest of CTCs with above features is found valuable for the subsequent academic researches or clinical studies (e.g. molecular mechanisms underlying cancer metastasis, cancer-related gene mutation, biomarker discovery, and particularly CTCs-based chemotherapy drug testing). These could both facilitate and accelerate scientists to develop new therapeutic solutions for future cancer care.
Conditions
Conditions (1)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Cell Carcinoma | — | UNRESOLVED | — |
Interventions
Interventions (1)
| Intervention | Type | Mapped drug | Match |
|---|---|---|---|
| CTCs isolation/purification and cell number expansion | Procedure | — | UNRESOLVED |
Design
Arms and outcomes
Arms (2)
- label
- Cancer for CTC culture
- description
- participants are cancer patients with transitional cell carcinoma
- interventionNames
- Procedure: CTCs isolation/purification and cell number expansion
- label
- Healthy for CTC culture
- description
- enroll participants without cancer
- interventionNames
- Procedure: CTCs isolation/purification and cell number expansion
Primary outcomes (6)
- measure
- the effect of cell culture model for the Cell viability
- timeFrame
- baseline
- description
- optimize the 3-D cell culture model to measure the Cell viability
Eligibility
Eligibility (as posted)
- Sex
- All
- Minimum age
- 20 Years
Show eligibility criteria text
Inclusion Criteria: 1. patients who agreed with the content of informed consent of the study protocol 2. patients who agreed the researcher to review the medical record 3. adults \>20 years old 4. Patients have the right to asked withdrawing from the trial at any timepoints 5. meet the following requirements (1)cancer participants:cancer patients with transitional cell carcinoma (2)healthy participants:participants without cancer Exclusion Criteria: 1. Patients who refused the collection of blood and the reviewing of medical record 2. The investigators suggest to withdraw
References
Publications (3)
- BACKGROUNDOppenheimer SB. Cellular basis of cancer metastasis: A review of fundamentals and new advances. Acta Histochem. 2006;108(5):327-34. doi: 10.1016/j.acthis.2006.03.008. Epub 2006 May 26. PMID 16730054
- BACKGROUNDMehlen P, Puisieux A. Metastasis: a question of life or death. Nat Rev Cancer. 2006 Jun;6(6):449-58. doi: 10.1038/nrc1886. PMID 16723991
- BACKGROUNDKonstantopoulos K, Thomas SN. Cancer cells in transit: the vascular interactions of tumor cells. Annu Rev Biomed Eng. 2009;11:177-202. doi: 10.1146/annurev-bioeng-061008-124949. PMID 19413512