Clinical trial · Interventional
Effects of Calorie Restriction and Cold Stimuli on Health-related Indicators, Cognitive and Motor Functions
Effects of 2-day and 6-day Calorie Restriction and Whole-body Cooling Strategies on Health-related Indicators, Cognitive and Motor Functions
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
The goal of this clinical trial was to identify if duration of acute calorie restriction (CR)/fasting or combination CR with cold stimuli have any effects on mental and physical health-related markers, and to clarify if different fasting strategies have any effect on cognitive and motor functioning efficiency in different genders. The main questions it aims to answer were: * Does fasting duration have any effect on fasting evoked responses? * Does cold interventions can modulate fasting evoked responses? * Does sex have any effect on acute fasting evoked responses? For the first part, participants were randomly classified into 4 groups: two experimental groups: 2-days of CR (0 kcal diet) and 6-days of CR (0 kcal diet), and two control groups: 2-days or 6-days usual diet. For the second part, participants were randomly assigned to undergo the following conditions: 2-days of CR with two 10-min whole-body cold-water immersions on separate days, 2-days of CR without cold-water immersion, 2-days without CR with two 10-min whole-body cold-water immersions on separate days, or 2-days of the usual diet without cold-water immersion in a randomized crossover fashion. Changes in anthropometric characteristics, perceived stress, metabolism, overall health (total blood count, sex hormones, etc.), psycho-emotional state, cognitive and motor functions were examined.
Conditions
Conditions (3)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Calorie Deficiency | — | UNRESOLVED | — |
| Cold Exposure | — | UNRESOLVED | — |
| Fasting | — | UNRESOLVED | — |
Interventions
Interventions (4)
| Intervention | Type | Mapped drug | Match |
|---|---|---|---|
| 2-days of usual diet with whole-body cold-water immersion | Other | — | UNRESOLVED |
| 2-days of zero calorie restriction and whole-body cold-water immersion | Other | — | UNRESOLVED |
| 2-days of zero calorie restriction without whole-body cold-water immersion | Other | — | UNRESOLVED |
| 6-days of calorie restriction | Other | — | UNRESOLVED |
Design
Arms and outcomes
Arms (6)
- type
- EXPERIMENTAL
- label
- 2-days of calorie restriction with whole body cooling
- description
- Healthy young subjects participated in a 2-day CR (0 kcal with water provided ad libitum ) with two 10-min whole body cooling practices on separate days. All participants were asked to not perform excessive sports activities while the research was ongoing, not to be involved in any temperature-manipulation program or extreme temperature exposure for ⩾3 months; do not use any medications that could affect experimental data.
- interventionNames
- Other: 2-days of zero calorie restriction and whole-body cold-water immersion
- type
- EXPERIMENTAL
- label
- 6-days of calorie restriction
- description
- Healthy young subjects participated in 6-day CR (0 kcal with water provided ad libitum). All participants were asked to not perform excessive sports activities while the research was ongoing, not to be involved in any temperature-manipulation program or extreme temperature exposure for ⩾3 months; do not use any medications that could affect experimental data.
- interventionNames
Eligibility
Eligibility (as posted)
- Sex
- All
- Minimum age
- 18 Years
- Maximum age
- 40 Years
Show eligibility criteria text
Inclusion criteria: * body mass index 19.5-29.9 kg/m2, * age 18-40 years, * no excessive regular moderate or vigorous physical activity, i.e., ≥3 times per week, * non-smokers, * no medications that could affect the results of experiment, * no history of any eating, oncological, metabolic, cardiovascular, skeletal, neuromuscular, pulmonary disorders or diseases, mental disabilities or conditions that could be worsened by exposure to acute cold (14°) water or fasting and affect experimental variables, * no history of alcoholism or dependence on psychotropic drugs, * no blood/needle phobia, * no involvement in any temperature-manipulation program or extreme temperature exposure for ⩾3 months, Exclusion criteria: smoking, were in a weight-reduction program, followed a low-carbohydrate diet.
References
Publications (3)
- DERIVEDSolianik R, Eimantas N, Dauge G, Brazaitis M. The effect of 6-day fasting on physical performance and neuromuscular control in healthy men. Sci Rep. 2026 Jun 20. doi: 10.1038/s41598-026-57312-9. Online ahead of print. PMID 42323358
- DERIVEDSolianik R, Eimantas N, Dauge G, Brazaitis M. Sex-related differences in fine motor skills and muscle strength control during short-term fasting. Sci Rep. 2026 Jan 3;16(1):4245. doi: 10.1038/s41598-025-34354-z. PMID 41484413
- DERIVEDSolianik R, Zidoniene K, Baranauskiene N, Brazaitis M. Fasting for 48 h induced similar glucose intolerance in both sexes despite greater perceived stress and decreased estradiol levels in females. Eur J Appl Physiol. 2024 May;124(5):1449-1459. doi: 10.1007/s00421-023-05378-y. Epub 2023 Dec 18. PMID 38108909