Establishment of Individualized Immunotherapy Strategy and Platform Based on Changes of Intestinal Microbiota

Inclusion Criteria:

1. Age: 18-80 years old, no gender limit;
2. BMI index 18.5-23.9kg/m2;
3. Patients with esophageal squamous cell carcinoma, gastric adenocarcinoma or gastrointestinal stromal tumor diagnosed by pathology;
4. Patients who intend to undergo individualized immunotherapy, who have not undergone surgery in the past;
5. Patients with advanced or unresectable upper gastrointestinal tumors according to clinical stage;
6. ECOG score: 0-1 points;
7. Estimated survival period ≥ 3 months;
8. All patients should have measurable or evaluable target lesions;
9. Able to eat a liquid diet or above; no complete obstruction or perforation of the digestive tract; no distant metastasis;
10. The main organs are functioning normally, that is, they meet the following standards:

(1) Routine blood examination standards must meet (no blood transfusion and blood products within 14 days, no correction with G-CSF and other hematopoietic stimulating factors):

1. HB≥80 g/L;
2. ANC≥1.5×109/L;
3. PLT≥100×109/L; (2) The biochemical inspection shall meet the following standards:

a) TBIL\<1.5×ULN; b) ALT and AST\<2.0×ULN; c) Serum Cr≤1.5×ULN or endogenous creatinine clearance\> 50 mL/min (Cockcroft-Gault formula); (3) Pulmonary function assessment: Pulmonary function is normal or mild to moderately abnormal (VC%\>60%, FEV1\>1.2L, FEV1%\>40%, DLco\>40%); (4) Cardiovascular function assessment: cardiac function grade Ⅰ～Ⅱ; 11. Have a certain degree of self-care ability and language comprehension ability;

Exclusion Criteria:

1. Patients with pathological types and primary foci that do not meet the inclusion criteria;
2. People who are known to be allergic to macromolecular protein preparations, or to immunological preparations and contrast agents and their preparation components;
3. Risk of digestive tract perforation;
4. Evidence of distant organ metastasis;
5. Surgical treatment (except biopsy), radiotherapy, chemotherapy, and molecular targeted therapy have been performed;
6. Have suffered from other malignant tumors;
7. History of serious lung or heart disease;
8. Have active infection or have fever of unknown cause \> 38.5℃ within 2 weeks prior to randomization (according to the judgment of the investigator, the subjects' fever due to tumor can be included in the study);
9. Significant active infection is known, or significant blood, renal, metabolic, gastrointestinal, or endocrine dysfunction is determined by the investigator;
10. A history of immunodeficiency, including HIV positive or other acquired or congenital immunodeficiency disease, or a history of organ transplantation;
11. The subject has active hepatitis B (HBV DNA ≥ 2000 IU/mL or 104 copies/mL), hepatitis C (positive for hepatitis C antibody and hcV-RNA higher than the detection limit of analysis method);
12. Those who received live vaccine within 3 months prior to treatment;
13. Patients with acute or chronic tuberculosis infection (positive t-spot test, suspicious tuberculosis foci on chest X-ray);
14. History of drug, drug or alcohol abuse (drinking ≥5 times a week, ≥2 liang of liquor each time, etc.);
15. The patient participated in clinical trials of other antineoplastic agents within 4 weeks;
16. No intravenous infusion;
17. Severe diarrhea in the past 2 months (≥3 watery stools per day for ≥3 days);
18. Severe constipation in the past 2 months (defecation ≤2 times per week, with difficulty defecating);
19. Used antibiotics for 3 days or more in the past 2 months;
20. Used proton pump stomach drugs, acid suppressants, mucosal protectants, opioid psychotropic drugs, hormones, immunosuppressants, cytotoxic drugs and other drugs within the past 2 months for 3 days or more;
21. Used probiotics, prebiotics or biosaccharides for 3 days or more in the past 2 months;
22. Abnormal thyroid function caused by cholecystitis, gastrointestinal ulcer, urinary tract infection, acute pyelonephritis, cystitis, hyperthyroidism and other diseases within the past 1 month;
23. Medical procedures such as gastrointestinal surgery, appendicitis surgery or enema or bowel cleansing have been performed within the past 1 year;
24. Lactose intolerance during pregnancy and lactation (female);
25. Patients with hypertension who cannot be reduced to the normal range by antihypertensive medication (systolic blood pressure \>140 mmHg, diastolic blood pressure \>90 mmHg);A history of unstable angina pectoris;Patients who were newly diagnosed with angina pectoris within 3 months before screening or had myocardial infarction within 6 months before screening;Arrhythmias (including QTcF: ≥450 ms for men and ≥470 ms for women) requiring long-term use of antiarrhythmic drugs and New York Heart Association classification ≥II cardiac insufficiency;
26. In the investigator's judgment, the subject has other factors that may cause him/her to be forced to terminate the study, such as other serious diseases (including mental illness) requiring combined treatment, seriously abnormal laboratory test values, family or social factors that may affect the subject's safety or the collection of test data;
27. The investigator determines other conditions that may affect the conduct of the clinical study and the determination of the study results;
28. Partners or first-degree relatives of research centre staff, researchers;