Donor-Derived Ex-Vivo Expanded Natural Killer Cell Infusions in Children and Young Adults With High Risk Acute Myeloid Leukemia Receiving Myeloablative HLA-Haploidentical Hematopoietic Cell Transplant

Inclusion Criteria:

* Age ≤ 25 years at time of enrollment
* High-risk AML, as defined by one of the following:
* AML in CR1 (defined as \<5% blasts in BM by morphology and flow cytometry) having at least one of these high-risk features:
* Mutations associated with high risk disease (Appendix A). Other high-risk features not explicitly stated in Appendix A can be considered after discussion/approval with the protocol chair/team
* MRD-positive at the end of Induction I chemotherapy (defined as flow cytometry ≥ 0.1% blasts)
* AML in ≥CR2 (defined by \<5% blasts in BM by morphology and flow cytometry)
* Recovery from prior cycle of chemotherapy as defined by an absolute neutrophil count ≥ 500/mm3
* AML secondary to select germline marrow failure disorders (with exception of Fanconi Anemia) may be eligible but require approval from Protocol Chairs prior to enrollment.
* Performance status ≥70% (Lansky for \<16 years; Karnofsky for ≥16 years)
* Adequate major organ system function as demonstrated by:
* Renal: Creatinine clearance (CrCl) ≥60 mL/min/1.73m2 by Cockcroft-Gault formula, Schwartz formula, or nuclear GFR study (Table 3)
* Hepatic: Total bilirubin \<2 mg/dL (unless due to Gilbert syndrome) and ALT and AST \< 5x ULN
* Cardiac: LVEF at rest ≥50% or SF ≥27% (by MUGA or ECHO)
* Pulmonary: DLCO, FEV1, and FVC ≥ 50% of predicted corrected for hemoglobin. For patients \<7 years of age or those unable to perform PFTs: O2 Sat \>92% on room air by pulse oximetry and on no supplemental O2 at rest
* The patient, patient's parent, guardian, or legal representative can provide written informed consent

Exclusion Criteria:

* Active extramedullary disease
* Unresolved/ongoing and serious viral, bacterial, or fungal infection despite appropriate treatment
* Positive pregnancy test in a female of child-bearing potential (FCBP)
* Inability to comply with medical therapy or follow-up
* Prior allogeneic transplant
* Patients with Fanconi Anemia and Down syndrome