The Gene Profiles of Primary and Heterogeneous Metastases of Colorectal Cancer

Inclusion Criteria:

1. Age ≥ 18 years old and ≤ 75 years old, both men and women;
2. Patients must have histologically confirmed metastatic colorectal adenocarcinoma and metastases can be punctured by interventional evaluation;
3. ECOG: 0 to 2;
4. Expected survival ≥ 12 weeks;
5. Absolute neutrophil count (ANC) ≥ 1.5 × 109 /L; Hemoglobin ≥ 8g / dL; Platelets ≥100×109/L;(According to the normal value of the clinical trial center)
6. Prothrombin time (PT) \< 1.5 times the upper normal limit and thrombin time (APTT) \< 1.5 times the upper normal limit;
7. Informed consent has been signed.

Exclusion Criteria:

1. Patients had surgery within three weeks will be excluded.
2. Patients have known or suspected brain metastases will be excluded.
3. Pregnant or nursing women will be excluded.
4. Patients with severe uncontrolled systemic disease, such as severe active infection, will be excluded.
5. Patients had other malignancies in the past 5 years will be excluded, except cervical carcinoma in situ or basal cell carcinoma of the skin.
6. Patients who were unable to complete the study or sign valid informed consent for medical, social or psychological reasons, which will be determined by researcher, will be excluded.
7. Organ transplant recipients who need immunosuppressive therapy will be excluded.
8. People have been known to be infected with immunodeficiency virus (HIV) or have been known to be serologically positive for HIV will be excluded.