Clinical trial · Observational
Utility of Primary Glioblastoma Cell Lines
Glioblastoma Lines as the Disease Model
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
In this study, the characterization of human malignant glioma cell lines is described. After mechanical and enzymatic digestion of glioblastoma human biopsies from Neuromed IRCCS Neurosurgery patients, the investigators analyzed the established cell lines by immunohistochemistry. The investigators have already characterized 10 cell lines and results revealed that not all cell lines are positive for glial fibrillary acidic protein (GFAP), but only one was positive: the so-called COGI cell line. Moreover, the expression of Isocitrate Dehydrogenase 1(IDH1) and alpha thalassemia/mental retardation syndrome X-linked protein (ATRX) was investigated in all established cell lines. COGI cell line was also positive for IDH1R132 mutation and for ATRX. The results of characterization were summarized in table 1.
Conditions
Conditions (2)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Brain Tumor | Brain Neoplasm | ALIAS | 0.90 |
| Tumor | Neoplasm | ALIAS | 0.90 |
Interventions
Interventions (0)
Data not yet available
Design
Arms and outcomes
Arms (0)
[]Primary outcomes (1)
- measure
- Establishment of primary glioblastoma cell lines
- timeFrame
- 2 years
- description
- Obtaining tumor biopsies to generate primary glioblastoma cell lines. Cells will also be used for phenotypic, genetic (IDH mutation and MGMT promoter methylation) and immunohistochemical characterization (e.g. glial markers GFAP, IDH1, P53, ATRX and proliferation marker Ki67), natural and synthetic drug screening in order to assess a dose-response correlation, or other molecular and cellular techniques.
Eligibility
Eligibility (as posted)
- Sex
- All
Show eligibility criteria text
Inclusion Criteria: • Patients with brain tumor: Glioblastoma (grade IV WHO) Exclusion Criteria: * Patients with other types of cancer * Patients with brain tumor recurrence
References
Publications (12)
- BACKGROUNDDolecek TA, Propp JM, Stroup NE, Kruchko C. CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2005-2009. Neuro Oncol. 2012 Nov;14 Suppl 5(Suppl 5):v1-49. doi: 10.1093/neuonc/nos218. No abstract available. PMID 23095881
- BACKGROUNDKleihues P, Louis DN, Scheithauer BW, Rorke LB, Reifenberger G, Burger PC, Cavenee WK. The WHO classification of tumors of the nervous system. J Neuropathol Exp Neurol. 2002 Mar;61(3):215-25; discussion 226-9. doi: 10.1093/jnen/61.3.215. PMID 11895036
- BACKGROUNDDiao W, Tong X, Yang C, Zhang F, Bao C, Chen H, Liu L, Li M, Ye F, Fan Q, Wang J, Ou-Yang ZC. Behaviors of Glioblastoma Cells in in Vitro Microenvironments. Sci Rep. 2019 Jan 14;9(1):85. doi: 10.1038/s41598-018-36347-7. PMID 30643153
- BACKGROUNDStupp R, Weber DC. The role of radio- and chemotherapy in glioblastoma. Onkologie. 2005 Jun;28(6-7):315-7. doi: 10.1159/000085575. Epub 2005 Jun 2. No abstract available. PMID 15933418
- BACKGROUNDArcella A, Oliva MA, Staffieri S, Aalberti S, Grillea G, Madonna M, Bartolo M, Pavone L, Giangaspero F, Cantore G, Frati A. In vitro and in vivo effect of human lactoferrin on glioblastoma growth. J Neurosurg. 2015 Oct;123(4):1026-35. doi: 10.3171/2014.12.JNS14512. Epub 2015 Jul 17. PMID 26186026
- BACKGROUNDArcella A, Oliva MA, Sanchez M, Staffieri S, Esposito V, Giangaspero F, Cantore G. Effects of hispolon on glioblastoma cell growth. Environ Toxicol. 2017 Sep;32(9):2113-2123. doi: 10.1002/tox.22419. Epub 2017 Jun 15. PMID 28618133
- BACKGROUNDArcella A, Oliva MA, Staffieri S, Sanchez M, Madonna M, Riozzi B, Esposito V, Giangaspero F, Frati L. Effects of aloe emodin on U87MG glioblastoma cell growth: In vitro and in vivo study. Environ Toxicol. 2018 Nov;33(11):1160-1167. doi: 10.1002/tox.22622. Epub 2018 Sep 15.