A Study of CD147-targeted CAR-T by Hepatic Artery Infusions for Very Advanced Hepatocellular Carcinoma

Inclusion Criteria:

1. Age ≥ 18 year and ≤ 65 years, both male and female.
2. Advanced hepatocellular carcinoma(HCC) patient, which is untreatable by surgery or local therapy, or has postoperative progressions, failed at least one and two line of standard systemic chemotherapy, and unwilling or intolerance to targeting therapy or immune-therapy of cancer.
3. The portal vein is not total occlusion, or collateral circulation has formed between hepatic artery and blocked portal vein.
4. Patient with measurable HCC focus defined by mRECIST.
5. Patient with histologically confirmed diagnosis of CD147+ hepatocellular carcinoma.
6. Adequate venous access for apheresis, and no other contraindications for apheresis.
7. Child-Pugh score ≤7.
8. Eastern Cooperative Oncology Group(ECOG) performance status of 0-2.
9. Patient with a life expectancy of greater than three months.
10. Patients must able to understand and be willing to sign an informed consent.

Exclusion Criteria:

1. Patients with fibrolamellar carcinoma of liver，mixed hepatocellular carcinoma or cholangiocarcinoma.
2. Patients with severe hypohepatia including jaundice, hepatic encephalopathy, refractory ascites or hepatorenal syndrome.
3. Patients with severe comorbidity, including any of the following.

   1. Unstable angina pectoris and/or congestive heart failure need hospitalization;
   2. Myocardial infarction or cerebrovascular accident (CVA) in the last 6 months;
   3. chronic obstructive pulmonary disease progressions or need hospitalization;
   4. severe cardiovascular, nervous system, hematological, gastrointestinal, endocrine diseases or metabolic disorders;
   5. autoimmune disease or immunodeficiency disease;
   6. acute bacterial infections or fungal infections needs intravenous injection of antibiotics during CAR-T cell therapy;
   7. tuberculosis not cured;
   8. other malignancies;
4. Patients who have received gene therapy, cell therapy or immune therapy.
5. Patients who have received organ transplantation.
6. Patients who have received treatment of targeted drugs, glucocorticoid or immunosuppressive drugs within 2 weeks before enrolling in clinical trial.
7. Patients who have received chemotherapy except for lymphocyte clearance within 2 weeks before enrolling in clinical trial.
8. Patients who have received radiotherapy.
9. Patients who did not recover to CTCAE(v5.0) grade 1 from adverse events （except hair）of previous anti-tumor therapy before enrolling in trial.
10. Syphilis test (TRUST) positive, Anti-HIV positive, Anti-HCV positive with HCV-RNA level higher than the lower limit of detection(LOD), or HBcAb positive with HBV-DNA level higher than the LOD.
11. Patients with following abnormalities:

    1. Absolute neutrophil count (ANC) \< 1.5E9/L, platelet(PLT) \< 50E9/L, or hemoglobin(HGB)\< 80 g/dL;
    2. Prothrombin time (PT), activated partial thromboplastin time (APTT) or international normalized ratio (INR) \> 1.5×ULN (upper normal value);
    3. Total bilirubin(TBIL) \> 2×ULN; ALT, AST or ALP\>5×ULN;
    4. Serum creatinine (Cr)≥1.5×ULN or glomerular filtration rate (GFR) \< 60 mL/min·1.73m\^2;
    5. left ventricular ejection fraction (LVEF) \< 50%;
12. Patients with a history of allergic reactions attributed to any agents or compounds involved in this study.
13. Patients with a history of mental disorders.
14. Patients with a history of drug abuse.
15. Pregnant and lactating women.
16. Patients of childbearing age who unwilling or unable to take birth control from during this study and 3 months post this study.
17. Patients who receive any other investigational agents within the 3 months before enrolling in this clinical trial.
18. Investigator considers not suitable for this trial.