Therapeutic Vaccine Plus PD-1 Knockout in Prostate Cancer Treatment

Inclusion Criteria:

* • Histologically confirmed prostate cancer (stage IV, according to NCCN Clinical Practice Guidelines in Oncology: Prostate Cancer, Version 2.2017)

  * Evidence of metastasis in the soft tissue and/or bone.
  * Progressive androgen independent castrate resistant prostate cancer.
  * Serum PSA ≥ 5.0 ng/mL
  * Estimated life expectancy ≥ 6 months.
  * Castrate level of testosterone (\< 50 ng/dL) achieved via medical or surgical castration.
  * Adequate hematologic, renal and liver function.

Exclusion Criteria:

* • Presence of known lung, liver, or brain metastases, malignant pleural effusions, or malignant ascites.

  * Presence of moderate to severe pain treating with opioid analgesics within 21 days prior to registration.
  * ECOG score ≥ 2.
  * Any other systemic therapy for prostate cancer (except for medical castration).
  * Participation in previous study using Provenge (Sipuleucel-T) or similar product.
  * Known pathologic long-bone fractures, imminent pathologic long-bone fracture (cortical erosion on radiography \> 50%) or spinal cord compression.
  * Known malignancies other than prostate cancer requiring active treatment within 6 months.
  * A requirement for systemic immunosuppressive therapy for any reason.
  * A history of allergic reactions attributed to compounds of similar chemical or biologic composition to this product or granulocyte-macrophage colony-stimulating factor.
  * Any infection requiring parenteral antibiotic therapy or causing fever (temp \> 100.5°F or \> 38.1°C) within 1 week prior to registration.
  * Any medical intervention or other condition which, in the opinion of the Principal Investigator could compromise adherence with study requirements or otherwise compromise the study's objectives.
  * Treatment with any of the following medications or interventions within 28 days of registration:

Systemic use of corticosteroids, External beam radiation therapy or surgery, Use of non-steroidal antiandrogens Dietary and herbal supplements, as well as alternative treatments that have evidence of hormonal and/or anticancer properties (e.g., prostate cancer (PC) -SPES or PC-SPEC) and saw palmetto, Megestrol acetate (Megace®), diethylstilbesterol (DES), or cyproterone acetate, ++Ketoconazole, 5-alpha-reductase inhibitors, Treatment with any other investigational product Treatment with chemotherapy High dose calcitriol \[1,25(OH)2Vitamin D\] (i.e., \> 0.5 mcg/day). Initiation or discontinuation of bisphosphonate therapy