Clinical trial · Observational
Comparison of Vitrification Effect Before or After In Vitro Maturation
Characterization of a Method of Fertility Preservation for Patients Diagnosed for a Cancer
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
Human oocyte cryopreservation is routinely used for fertility preservation of women who will be exposed to gonadotoxic effect of cancer treatment. After ovarian stimulation, matured oocytes are vitrified. However, this strategy cannot always be used, particularly for hormone-sensitive cancer or when ovarian stimulation is not possible. An approach including immature oocytes and in vitro maturation (IVM) could be considered in these cases. While some qualitative analysis of oocytes vitrified before or after IVM suggest that vitrification should be performed after IVM, little is known about vitrification effects on actin and tubulin cytoskeleton and kinetic of maturation of human ovocytes. To answer to this question, Investigator performed quantitive analyses comparing matured oocytes from three different groups: vitrified before IVM or after IVM and non-vitrified oocytes. Non-vitrified matured oocytes were used as a control. Different parameters have been analysed during maturation and in matured oocytes.
Conditions
Conditions (2)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Oocytes Matured in Vitro | — | UNRESOLVED | — |
| Oocytes Vitrified at Prophase-I Stage | — | UNRESOLVED | — |
Interventions
Interventions (1)
| Intervention | Type | Mapped drug | Match |
|---|---|---|---|
| Oocyte vitrification | Other | — | UNRESOLVED |
Design
Arms and outcomes
Arms (3)
- label
- Immature oocytes vitrified before In Vitro Maturation
- description
- Immature oocytes were vitrified using closed system vitrification. After warming, they were cultured during 36 hours in IVM medium and fixed for cellular analysis
- interventionNames
- Other: Oocyte vitrification
- label
- Immature oocytes cultured in vitro before vitrification
- description
- Immature oocytes were cultured in vitro in IVM medium during 36 hours. After IVM, they were vitrified. After warming, they were fixed for cellular analysis.
- interventionNames
- Other: Oocyte vitrification
- label
- Fresh oocytes
- description
- Immature oocytes were cultured in vitro in IVM medium during 36 hours and subsequently, fixed for cellular analysis.
- interventionNames
Eligibility
Eligibility (as posted)
- Sex
- Female
- Minimum age
- 18 Years
- Maximum age
- 37 Years
Show eligibility criteria text
Inclusion Criteria: * \- ICSI treatment * Immature oocytes * \< 37 years old Exclusion Criteria: * \- Polyckistic Ovarian Syndrome * Endometriosis * Ovulatory disease
References
Publications (0)
Data not yet available