The Effect of Menstrual Cycle On IL-6, TNF-α And Periodontal Status

Aim: Women's menstrual cycle is controlled by the secretion of sex hormones. Sex steroid hormones are thought to be affect to periodontal tissues, wound healing, periodontal disease progression and bone turn over. The aim the study is to evaluate clinical periodontal parameters and interleukin- 6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels in gingival crevicular fluid (GCF) in menstrual cycle. Material and methods: Twenty-five patients were included in this study clinical periodontal parameters and levels of IL-6 and TNF α in gingival crevicular fluid were evaluated at three periods of menstrual cycle (menstruation day (MD)-ovulation day(OD)-premenstruation day (PmD)). Levels of IL-6 and TNF α in gingival crevicular fluid were determined by ELISA.

Sex

Female

Minimum age

20 Years

Maximum age

29 Years

Inclusion Criteria:Inclusion criteria were:

1. premenopausal women, age 20-35 to years;
2. normal and steady menstrual cycle (25 to 31 days long);
3. available for clinical examination;
4. non-smoking;
5. antibiotic therapy or use of nonsteroidal anti-inflammatory drugs within the past 6 months;
6. healthy systemic status;
7. normal body mass index;
8. healthy periodontium.

Exclusion Criteria: Exclusion criteria were as follows:

1. pregnancy;
2. use of oral contraceptives;
3. metabolic or systemic condition that might affect the periodontium;
4. antibiotic therapy or use of nonsteroidal anti-inflammatory drugs within the past 6 months;
5. periodontitis;
6. smoking and
7. with the diagnosis of polycystic over or hyperandrogenism according to Rotterdam diagnostic criteria