Clinical trial · Observational
Epigenetic Integrity of Spermatozoa in Patients With Germinal Testicular Tumours
Epigenetic Integrity of Spermatozoa in Patients With Germinal Testicular Tumours: Potential Risks and Consequences for the Conceptus
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
Recent data suggest that sperm cells carry an epigenetic message during spermatogenesis and that this message is crucial for the future development of the embryo. This epigenetic signature is notably represented by methylation of genes subjected to imprinting (GSI) and the methylation of transposable elements (TE). Data on the maintenance of the imprint and of the control of TE accompanying human gametogenesis in a context of adult germinal testicular cancers, seminomas, are extremely fragmentary for tumour tissues and inexistent for gametes. The aim of this study is to determine whether patients with seminomas in comparison with fertile men carry a higher risk of presenting epigenetic alterations affecting their gametes. This study is based on the use of an existing collection of biological samples. 90 samples will be selected and split into 3 groups: * Group 1: 30 sperm samples from patients with seminomatous testicular tumours * Group 2: 30 sperm samples from fertile patients * Group 3: 30 sperm samples from infertile patients After treatment of the samples (thawing, cell sorting and removal of cryoprotectants), they will be analysed.
Conditions
Conditions (1)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Seminomas | Seminoma | ONTOLOGY_EXACT | 0.90 |
Interventions
Interventions (4)
| Intervention | Type | Mapped drug | Match |
|---|---|---|---|
| Analysis of methylation for the whole genome (sub-population of 15 samples from group 1) | Other | — | UNRESOLVED |
| Analysis of methylation profiles of numerous GSI and TE | Other | — | UNRESOLVED |
| Extraction genomic DNA | Other | — | UNRESOLVED |
| Method of protein detection by immunostaining and flow cytometry | Other | — | UNRESOLVED |
Design
Arms and outcomes
Arms (3)
- label
- testicular tumours
- interventionNames
- Other: Extraction genomic DNA
- Other: Analysis of methylation profiles of numerous GSI and TE
- Other: Analysis of methylation for the whole genome (sub-population of 15 samples from group 1)
- Other: Method of protein detection by immunostaining and flow cytometry
- label
- fertile
- interventionNames
- Other: Extraction genomic DNA
- Other: Analysis of methylation profiles of numerous GSI and TE
- Other: Method of protein detection by immunostaining and flow cytometry
- label
- infertile
- interventionNames
- Other: Extraction genomic DNA
- Other: Analysis of methylation profiles of numerous GSI and TE
- Other: Method of protein detection by immunostaining and flow cytometry
Primary outcomes (1)
Eligibility
Eligibility (as posted)
- Sex
- Male
- Minimum age
- 18 Years
Show eligibility criteria text
Inclusion Criteria: * biological samples from persons who have provided written consent * biological samples of patients over 18 years old * with testicular tumours/Fertile/Infertile * Matched for age with patients suffering from testicular tumours Exclusion Criteria: * biological samples of persons without national health insurance cover * biological samples from patients with chronic hepatitis B or C or HIV infection
References
Publications (0)
Data not yet available