Clinical trial · Observational
Autophagy Bladder Cancer
Identification of Novel Autophagy Markers in Bladder Cancer Patients
NCT03254888CI-TRIAL-00045852unknownClinicalTrials.gov clinicaltrialsProvenance
- Source
- ClinicalTrials.gov
- Retrieved
- Sep 8, 2026
- Layer
- normalized (units and labels harmonized; values unchanged)
- Run
- ING-CLINICALTRIALS-20260908-000001
Summary
Brief summary (as posted)
• Bladder cancer is the most common malignancy of the urinary tract. It represents the 7th most commonly diagnosed cancer in male population worldwide and drops to the 11th when both genders are considered . According to the American cancer society's estimates of bladder cancer in 2017, the number of the new cases of bladder cancer is 79,030, and the mortality figures reached 16,870 .
Conditions
Conditions (1)
Free-text conditions as registered, with the CancerIndex entity they were reconciled to and the match type.
| Condition (as posted) | Mapped entity | Match | Confidence |
|---|---|---|---|
| Bladder Cancer | Malignant Bladder Neoplasm | CURATED_EXACT | 0.92 |
Interventions
Interventions (0)
Data not yet available
No intervention recorded.
Design
Arms and outcomes
Arms (4)
- label
- Low Grade group
- description
- • 50 tumor tissue samples from patients with Low Grade Bladder Cancer undergoing either trans urethral resection of bladder tumor or Radical Cystectomy , The followings markers must be estimated : 1. Autophagy markers: * ( Atg7) level using (quantitative real time polymerase chain reaction). * (LC3A)level using immunohistochemistry . 2. ER-stress marker: (ATF6) level using ELISA(Enzyme Linked Immuno sorbent Assay) 3. Oxidative stress Marker:(MDA)using chemical method 4. Apoptotic marker:(caspase 3) using(quantitative real time polymerase chain reaction) .
- label
- High Grade group
- description
- • 50 tumor tissue samples from patients with High Grade Bladder Cancer undergoing either trans urethral resection of bladder tumor or Radical Cystectomy, The followings markers must be estimated : 1. Autophagy markers: * ( Atg7) level using (quantitative real time polymerase chain reaction). * (LC3A)level using immunohistochemistry . 2. ER-stress marker: (ATF6) level using ELISA(Enzyme Linked Immuno sorbent Assay) 3. Oxidative stress Marker:(MDA)using chemical method 4. Apoptotic marker:(caspase 3) using (quantitative real time polymerase chain reaction) .
- label
- Safety margin group
- description
- • 50 normal bladder urothelial tissue samples from the safety margin around the tumor(0.5cm to the tumor), The followings markers must be estimated : 1. Autophagy markers: * ( Atg7) level using (quantitative real time polymerase chain reaction). * (LC3A)level using immunohistochemistry . 2. ER-stress marker: (ATF6) level using ELISA(Enzyme Linked Immuno sorbent Assay) 3. Oxidative stress Marker:(MDA)using chemical method 4. Apoptotic marker:(caspase 3) using (quantitative real time polymerase chain reaction).
Eligibility
Eligibility (as posted)
- Sex
- All
Show eligibility criteria text
Inclusion Criteria: * 1)patients confirmed histopathologically to have bladder cancer. 2) Both sexes. 3) Patients who will accept to participate in the study. Exclusion Criteria: * Patients with past history of Bladder Cancer with previous chemotherapy or any other types of cancer in the last 5 years.
References
Publications (10)
- RESULTWoldu SL, Bagrodia A, Lotan Y. Guideline of guidelines: non-muscle-invasive bladder cancer. BJU Int. 2017 Mar;119(3):371-380. doi: 10.1111/bju.13760. Epub 2017 Jan 24. PMID 28058776
- RESULTMathew R, Karantza-Wadsworth V, White E. Assessing metabolic stress and autophagy status in epithelial tumors. Methods Enzymol. 2009;453:53-81. doi: 10.1016/S0076-6879(08)04004-4. PMID 19216902
- RESULTKondo Y, Kanzawa T, Sawaya R, Kondo S. The role of autophagy in cancer development and response to therapy. Nat Rev Cancer. 2005 Sep;5(9):726-34. doi: 10.1038/nrc1692. PMID 16148885
- RESULTMizushima N. Autophagy: process and function. Genes Dev. 2007 Nov 15;21(22):2861-73. doi: 10.1101/gad.1599207. PMID 18006683
- RESULTLin YC, Lin JF, Wen SI, Yang SC, Tsai TF, Chen HE, Chou KY, Hwang TI. Inhibition of High Basal Level of Autophagy Induces Apoptosis in Human Bladder Cancer Cells. J Urol. 2016 Apr;195(4 Pt 1):1126-35. doi: 10.1016/j.juro.2015.10.128. Epub 2015 Oct 28. PMID 26519656
- RESULTMaiuri MC, Zalckvar E, Kimchi A, Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 2007 Sep;8(9):741-52. doi: 10.1038/nrm2239. PMID 17717517
- RESULTYang ZJ, Chee CE, Huang S, Sinicrope FA. The role of autophagy in cancer: therapeutic implications. Mol Cancer Ther. 2011 Sep;10(9):1533-41. doi: 10.1158/1535-7163.MCT-11-0047. Epub 2011 Aug 30. PMID 21878654
- RESULTSivridis E, Koukourakis MI, Mendrinos SE, Touloupidis S, Giatromanolaki A. Patterns of autophagy in urothelial cell carcinomas--the significance of "stone-like" structures (SLS) in transurethral resection biopsies. Urol Oncol. 2013 Oct;31(7):1254-60. doi: 10.1016/j.urolonc.2011.12.016. Epub 2012 Jan 24.