Study Evaluating the Efficacy and Safety With CAR-T for Relapsed or Refractory Neuroblastoma in Children

Inclusion Criteria:

1. Up to diagnostic criteria for relapsed or refractory neuroblastoma or high-risk patients，including:

   * Relapsed neuroblastoma : Children diagnosed with neuroblastoma who after standard treatment and remission, present lesions again and cannot reach complete remission with surgery.
   * Refractory neuroblastoma : ① Untreated patients that do not have to reach completes remission after 4 courses of chemotherapy in accordance with standard regimens nor reach complete remission with surgery. ② High-risk patients : Who have cell genetic variation, such as MYCN amplification or bone marrow metastasis.
2. Relapsed or Refractory Neuroblastoma: Target, of which expression may be intervened , discovered with Immunohistochemistry can be selected (GD2 +) (more than 50% of tumor cells is at least 2+ , adopting anti-GD2-mAb14G2a ).
3. Age: 1\~14 years old of age at the time of enrollment, male or female.
4. Physical condition is good: ECOG score reaches 0 to 2 points.
5. Body weights greater than or equal to 10 kg.
6. White blood cell counts acuity≥ 1.0 x10\^9 / L.
7. Estimated survival times \> 90 days.
8. Voluntary participation, good compliance, can cooperate with the experimental observation and signed an informed consent form.

Exclusion Criteria:

1. Positive pregnancy tests.
2. Uncontrolled infection.
3. HIV infection, hepatitis B or C activity period.
4. Patients who need long-term immunosuppressive therapy (Such as allergies, autoimmune diseases, GVHD, etc.)
5. Combined activity of the central nervous system malignant tumor invasion.
6. Abnormal coagulation function, patients with severe thrombosis.
7. Organ failure

   1. Heart：class Ⅱ or above.
   2. Liver：class Ⅱ or above( Refer to Classification of Wuhan Conference (1983)).
   3. Kidney: The second stage of renal insufficiency or above.
   4. Lung: class Ⅱdecreased slightly or above.
   5. Brain: The central nervous system transfer or have active lesions.
8. Patients who have participated in other clinical trials or other clinical trials in the past 30 days.
9. The researchers believe that the patient is not suitable to participate in the study.