The Effect of Vitamin D Supplementation Among Overweight Jordanian Women With Polycystic Ovary Syndrome (PCOS)

Inclusion Criteria:

* Female gender.
* Aged between 18 and 49 years old.
* Ethnic group (Caucasian, Middle-eastern).
* Overweight (BMI 25-30 kg\^m2).
* Diagnosed with Polycystic ovary syndrome according to Rotterdam criteria (Rotterdam SHRE-ASRM Sponsored Polycystic ovary syndrome consensus workshop group, 2004).
* Diagnosed with hypovitaminosis D (serum 25(OH)D level \< 20 ng/mL).
* Inadequate dietary intake of vitamin D (\<600 IU/day or \<15μg/day).
* Physical examination being assessed and accepted by the attending physician.
* Systolic blood pressure within the normal range (90-140 mmHg).
* Diastolic blood pressure within the normal range (60-90 mmHg).
* Heart rate within the normal range (60-100 BPM).
* Oral body temperature within the normal range (35.9 - 37.6 Cᵒ).
* Normal complete blood count , Liver Function enzymes test , Aspartate Transaminase (AST) , Alanine Transaminase (ALT) and Kidney function tests , Blood Urea Nitrogen (BUN) and Serum Creatinine (SrCr).
* Participant is willing and able to give informed consent for participation in the study.
* Able and willing to comply with all study requirements.

Exclusion Criteria:

* Female participants who are pregnant, lactating or planning pregnancy during the course of the study.
* Ethnic group: non Caucasian.
* Females aged \<18 or \>49 years old.
* Underweight, normal body weight ,Body Mass Index (BMI) \< 25 kg\^m2
* Obese or morbidly obese (BMI \> 30 kg/m2)
* Diagnosis with type 1 or type 2 diabetes mellitus, hypothyroidism, hyperthyroidism, liver disease, renal dysfunction, cardiovascular diseases, androgen-secreting tumor, Cushing syndrome, congenital adrenal hyperplasia, hyperprolactinemia, and/or virilism.
* Known history or presence of food allergies or intolerance (e.g dairy products or gluten-containing foods), or any known condition that could interfere with the absorption, distribution, metabolism, or excretion of drugs.
* History of drug or alcohol abuse, smoking of 10 cigarettes or more (or equivalent) per day.
* Participants who took medications known to affect metabolic parameters, such as metformin and corticosteroid drugs, vitamin D and calcium.
* Adequate dietary intake of vitamin D (600 IU/day or 15μg/day or more).
* Participation in another clinical or bioequivalence study within 90 days prior to the start of this study period.
* Participants with abnormal Electrocardiogram (ECG).
* Participants with any abnormal laboratory results excluding \[ 25(OH)D, Creatinine (Cr), Calcium (Ca), phosphorus (PO4), C-reactive protein(CRP) , triglyceride , High Density Lipoprotien Cholesterol (HDL-C), Low Density Lipoprotien Cholesterol (LDL-C), total cholesterol (TC)/HDL-C ratio, fasting insulin , fasting blood glucose, oral glucose tolerance test, impaired glucose tolerance, Progesterone, total testosterone, sex hormone binding globulin, parathyroid hormone and free androgen index\].