Autologous Cytomegalovirus (CMV) Specific CD8+ T Cells as Treatment for CMV Reactivation

Inclusion Criteria:

1. Patients must have received an allogeneic stem cell transplant from any donor, as treatment for a haematological malignancy.
2. HLAA0201 positive at one allele
3. CMV seropositive
4. The patient must be willing and capable of donating lymphocytes for CMVspecific CD8+ T cell selection using apheresis techniques
5. The patient must be in complete remission with no evidence of circulating blasts or other malignant cells
6. Patient must be fit to undergo leukapheresis
7. Patients must have signed an informed consent form before undergoing LP prior to alloSCT

Indications for infusion of autologous CMV specific CD8+ Tcells:

* Therapeutic: CMV disease following allogeneic stem cell transplantation
* Preemptive: CMV reactivation (by CMV DNA PCR)
* autologous CMV specific CD8+ T-cells must be infused into the patient no later than 72 following CMV reactivation.
* Steroids should be withdrawn at least 1 week before the infusion of CMVspecific CD8+ T-cell
* Patients must have signed an informed consent form before the infusion of autologous CMV specific CD8+ T-cells

Exclusion Criteria:

1. Patient CMV seronegative
2. No informed consent
3. Patient positive at the time of LP for one of the following infectious agents: HIV, HBV, HCV,Syphilis, HTLV 1 and 2
4. Patient with circulating leukemic blasts at the time of LP

Exclusion criteria for infusion of autologous CMV specific CD8+ T cells:

Severe GvHD (grade IIII-V) requiring full dose immunosuppressive treatment