Arginine Feeding: a Novel Strategy to Improve Protein Metabolism in Cancer and the Response to Surgery

Muscle catabolism is a major problem in cancer patients undergoing surgery as it negatively affects post-operative recovery. Recent evidence exists that protein metabolic changes are already apparent in cancer before muscle wasting is being present. In line, patients with breast cancer, generally characterized by a normal nutritional status, were recently found to be arginine deficient. Arginine deficiency in cancer can be explained by: 1) Reduced arginine availability, due to exhaustion of endogenous (muscle) sources of arginine 2) Enhanced arginine catabolism, due to conversion of arginine by arginase, which is abundant in tumors. Protein is the most important endogenous source of arginine. Arginine deficiency will lead to a negative feedback loop in cachexia by promoting protein breakdown in an attempt to restore plasma arginine levels. We hypothesize that pre-operative arginine supplementation in breast cancer patients diminishes the occurrence of muscle wasting after surgery by 1) normalizing arginine availability pre-operatively, resulting in conservation of protein, 2) diminishing the catabolic effects of surgery by supplying exogenous arginine for the post-operative response, 3) enhancing the anabolic capacity to feeding through supplying substrate for protein synthesis.

Sex

Female

Minimum age

30 Years

Inclusion Criteria:

Cancer groups (for aims 1 and 2)

1. Recently diagnosed (up to 4 weeks prior to treatment for cancer) with stage I, II or III invasive breast cancer
2. Undergoing mastectomy
3. Age greater than 30 years
4. Ability to sign informed consent
5. Good performance status defined by ECOG scale 0,1 or 2 (see CRF performance status)

Control group (for aim 1)

1. Age greater than 30 years
2. Undergoing prophylactic mastectomy
3. Ability to sign informed consent
4. Good performance status defined by ECOG scale 0,1 or 2 (see CRF performance status)

Exclusion Criteria:

All groups (aim 1 and 2)

1. Body weight loss of greater than 10% in the past 3 months
2. Previous anti-cancer therapy (e.g. chemotherapy or radiotherapy) or surgery less than 4 weeks prior to the experiment
3. Diagnosed diabetes type I or II
4. Untreated metabolic diseases including liver or renal disease
5. Any documented autoimmune disease
6. Use of corticosteroids, beta-antagonists or nitrovasodilators
7. Use of supplements enriched with amino acids
8. Presence of acute illness or metabolically unstable chronic illness
9. Unstable heart disease requiring therapy or recent myocardial infarction (less than 1 year)
10. Current alcohol or drug abuse (ETOH more than 2 servings per day)
11. Allergy/intolerance to any of the ingredients of the study products
12. Any other condition deemed by the PI and the study physician as exclusion or that interferes with proper conduct of the study/ safety of the patient.

• DERIVEDEngelen MPKJ, Klimberg VS, Allasia A, Deutz NEP. Major surgery diminishes systemic arginine availability and suppresses nitric oxide response to feeding in patients with early stage breast cancer. Clin Nutr. 2018 Oct;37(5):1645-1653. doi: 10.1016/j.clnu.2017.07.019. Epub 2017 Aug 5. PMID 28826699